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It was previously shown that human myotubes release membrane microparticles with an average diameter of 150 nm in response to an increase in intracellular Ca concentration.However, little is known about the characteristics of muscle-derived membrane microparticles (MMMs) and their biological functions.

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Further work to explore the biological significance of these MMMs in cell-cell signaling and skeletal muscle pathologies such as muscular dystrophy and insulin resistance are presently underway.

Funded by: A*STAR Singapore: There is increasing interest in the influence of microparticles (MP) on coagulation and inflammatory responses in trauma.

Quantitative proteomic analyses revealed that a selective repertoire of CD81-associated molecules, including Rac, was diminished in the exosomes derived from CD81-deficient animals. mi R-1289 and “zipcode”-like sequence enrich m RNAs in microvesicles Mehmet Fatih Bolukbasi, Arda Mizrak, Gokhan Baris Ozdener, Sibylle Madlener, Thomas Ströbel, Erdogan Pekcan Erkan, Jian-Bing Fan, Xandra O. We found s MVs to be released in higher numbers than c MVs, typically 10-fold higher numbers, in the same time frame, and where the stress factor was a pharmacological agent, the microvesiculation was an attempt to release this chemical stress factor.

Our data provide a firm evidence that insertion into TEMs is critical for the inclusion of these ligands into exosome structure. Breakefield and Okay Saydam Molecular Neuro-Oncology Research Unit, Department of Pediatrics, Medical University of Vienna, Austria Email: [email protected] intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles are still unknown. Using a mass sensing technique, the s MVs were released over a 15-minutes period after stimulation.

Proteins interacting with TEM receptors in cytoplasmic regions presented a considerable degree of overlap, although some highly specific CD81 tetraspanin ligands, such as Rac GTPase, were detected. Characterization of microvesicles released from cells constitutively and upon stimulation Dan Stratton University College London, Institute for Women's Health, Maternal and Fetal Medicine, London, United Kingdom Email: [email protected] Constitutively released microvesicles (c MVs) are released as a part of normal cell physiology.